Revealing biases inherent in recombination protocols
نویسندگان
چکیده
منابع مشابه
Revealing biases inherent in recombination protocols
BACKGROUND The recombination of homologous genes is an effective protein engineering tool to evolve proteins. DNA shuffling by gene fragmentation and reassembly has dominated the literature since its first publication, but this fragmentation-based method is labor intensive. Recently, a fragmentation-free PCR based protocol has been published, termed recombination-dependent PCR, which is easy to...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2007
ISSN: 1472-6750
DOI: 10.1186/1472-6750-7-77